ArrowH2S™ Complete Hydrogen Sulfide Measurement System

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ArrowH2S™ Complete Hydrogen Sulfide Assay System Rapidly and Accurately Measure H2S Directly in 96 Well Plates

Measure H2S in the following bio samples:

Plasma
Whole blood
Tissue homogenates
Cell Cultures
Cell Lysates
Urine

Click to See The ArrowH2S™ Story

Unique features of the ArrowH2S™ Hydrogen Sulfide Measurement System

Measure H2S directly in a 96 well plate
Simple sample prep- 3 step sample prep done directly in the 96 well plate
1. Add 40 microliters of sample to 96 well plate
2. Pipette additional 40 microliters of H2S reagent into 96 well plate
3. Dip H2S micro electrode into 96 well plate, stir slightly with electrode, wait 2 to 3 minutes and read H2S concentration on H2S analyzer display or your PC
Low detection limit - 1 uM (micro Molar) to maximum of 300 uM
Cost effective - significant savings over other techniques such as fluorescence, spectrophotometry, or HPLC- no capital equipment to buy
Use directly with your PC or laptop - Connects directly to your USB port for logging, graphing, and data recording directly on your PC
No known interferences - both proteins and these common anions WILL NOT interfere PO4-3, NO3-, NO2-, Cl-, I-, Br-, SO4=, SO3=, PO4-3, C2H3O2-

Each ArrowH2S™ system performs the following tasks:

Measures - Measures H2S using our sophisticated H2S analyzer and unique micro H2S ion electrode
Logs - Our exclusive software records details about your measurements straight to your PC for easy analysis
Graphs - Our exclusive software charts your data onto a graph so you can easily view and compare data

Ordering Information
ArrowH2S™ Micro Hydrogen Sulfide Measurement System

System includes micro H2S electrode, H2S analyzer with digital output to your PC or laptop, and complete software program for monitoring, recording, and logging H2S concentration data.

Item number:	ISM-146H2S-XS
Regular Price:	$1,750.00

The ArrowH2S™ System Story

Studies over the past decade have shown Hydrogen sulfide (H2S) to have a significant signaling role in biomedicine and biology. In particular, H2S, which is endogenously synthesized in mammals, was found to act as a vasodilator leading to dilation of blood vessels and the lowering of blood pressure and has been shown to have a protective effects on various cardiovascular conditions such as heart failure induced by myocardial ischemia. It has recently been shown to even have a greater role in human physiology by also acting as a gasotransmitter in the brain, respiratory system, kidneys, gastrointestinal system, and skeletal and smooth muscle. It has also been recently shown to have additional beneficial effects by acting as a scavenger for reactive oxygen species (ROS).

A major problem in any study of this gaseous signaling molecule is the tedious, labor intensive, time consuming, and sometimes inherent inaccuracies in measuring H2S concentration in biological media. Fluorescence (various fluorophores) , spectrophotometric methods (methylene blue reaction), gas chromatography, HPLC (monobrombimane assay), chemiluminescence, and polarographic techniques which are currently used to assay bio samples for H2S can not only suffer from significant interferences, but also require complex multi step sample prep procedures which can lead H2S loss from the sample.

In order to simplify the H2S assay procedure, reduce analysis time, while at the same time improving accuracy, Lazar Research Laboratories, Inc. with its decades of micro chemical sensor development experience, has spent a substantial amount of research time and effort to develop a unique micro solid state H2S electrode sensor which can be dipped directly into a 96 well plate or other micro sample container such as a microcentrifuge tube, and measure H2S concentration in less than 3 minutes without any known interferences. Concentration range for the H2S assay system is 1 uM (micro Molar) to 300 uM.

The testing procedure is quick and straight forward. You just pipette or add 40 microliters of the sample into a 96 well plate and then pipette an additional 40 microliters of a single reagent into the 96 well plate. Dip the micro H2S electrode into the 96 well plate, stir slightly by moving electrode, and after only 2 to 3 minutes read the H2S concentration on the H2S analyzer display or your PC. Rinse H2S electrode with DI water, dry gently using paper wiper, and proceed with the next sample. Its that simple.

For specific questions or to receive printed literature, send your request by email to service@lazarlab.com or call us at 814-870-9309, or fax us at 914-810-8660.
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